50-271-8553
VEGFB Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-271-8553-Each-of-1 | In Stock | ₹ 1,75,552.50 |
50-271-8553 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,75,552.50
GST (18%): ₹ 31,599.45
Total Price: ₹ 2,07,151.95
Antigen
VEGFB
Classification
Polyclonal
Conjugate
Biotin
Gene
VEGFB
Gene Alias
flt-1/VEGFR1 ligand; vascular endothelial growth factor B; vascular endothelial growth factor B167 precursor; vascular endothelial growth factor B186 precursor; vascular endothelial growth factor-B; Vegfb; VEGF-B; VEGFL; VEGF-related factor; Vrf
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
7423
Content And Storage
-20°C
Isotype
IgG
Applications
Western Blot, ELISA
Concentration
0.1-1.0 mg/mL
Formulation
PBS with no preservative
Gene Accession No.
P49765
Gene Symbols
VEGFB
Immunogen
E.coli-derived Recombinant Human VEGF-B
Quantity
500μg
Primary or Secondary
Primary
Target Species
Human
Form
Lyophilized
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Description
- AA Sequence of recombinant protein: PVSQPDAPGH QRKVVSWIDV YTRATCQPRE VVVPLTVELM GTVAKQLVPS CVTVQRCGGC CPDDGLECVP TGQHQVRMQI LMIRYPSSQL GEMSLEEHSQ CECRPKKKDS AVKPDSPRPL CPRCTQHHQR PDPRTCRCRC RRRSFLRCQG RGLELNPDTC RCRKLRR
- Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF-B
- Anti-Human VEGF-B-specific antibody was purified by affinity chromatography and then biotinylated
- Sandwich ELISA: To detect Human VEGF-B by sandwich ELISA (using 100 μL/well) a concentration of 0.25-1.0 μg/mL of this antibody is required
- This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human VEGF-B (500-P267) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human VEGF-B
- Western Blot: To detect Human VEGF-B by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
- When used in conjunction with compatible development reagents the detection limit for Recombinant Human VEGF-B is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
- 500-P267BT-1MG will be provided as 2 x 500 μg Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors
- VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis
- VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF
- There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186
- VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted
- VEGFB stimulates endothelial cell proliferation
- VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2
- Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas
- It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis
- The genes that encode VEGFB have been mapped to chromosome 11q13.