50-271-8516
Epo Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-271-8516-Each-of-1 | In Stock | ₹ 1,75,552.50 |
50-271-8516 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,75,552.50
GST (18%): ₹ 31,599.45
Total Price: ₹ 2,07,151.95
Antigen
Epo
Classification
Polyclonal
Conjugate
Biotin
Gene
EPO
Gene Alias
EP; EPO; Epoetin; erythropoietin; MGC138142; MVCD2
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
2056
Content And Storage
-20°C
Isotype
IgG
Applications
Western Blot, ELISA
Concentration
0.1-1.0 mg/mL
Formulation
PBS with no preservative
Gene Accession No.
P01588
Gene Symbols
EPO
Immunogen
CHO cells-derived Recombinant Human EPO
Quantity
500μg
Primary or Secondary
Primary
Target Species
Human
Form
Lyophilized
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Description
- AA Sequence of recombinant protein: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR
- Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human EPO
- Anti-Human EPO-specific antibody was purified by affinity chromatography and then biotinylated
- Sandwich ELISA: To detect Human EPO by sandwich ELISA (using 100 μL/well), a concentration of 0.25-1.0 μg/mL of this antibody is required
- This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EPO (500-P318) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human EPO
- Western Blot: To detect Human EPO by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/mL
- When used in conjunction with compatible development reagents, the detection limit for Recombinant Human EPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
- 500-P318BT-1MG will be provided as 2 x 500 μg Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors
- EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin
- The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood
- EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions
- Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.