Artemin Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Artemin
• Anti-Human Artemin-specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect hArtemin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Artemin (500-P245) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hArtemin
• Western Blot: To detect hArtemin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant hArtemin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P245BT-1MG will be provided as 2 x 500 μg The protein encoded by this gene is a member of the glial cell line-derived neurotophic factor (GDNF) family of ligands which are a group of ligands within the TGF-beta superfamily of signaling molecules
• GDNFs are unique in having neurotrophic properties and have potential use for gene therapy in neurodegenerative disease
• Artemin has been shown in culture to support the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons
• Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons
• This protein is a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor
• Multiple transcript variants encoding different isoforms have been found for this gene.