50-271-8509
Amphiregulin Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-271-8509-Each-of-1 | In Stock | ₹ 1,75,552.50 |
50-271-8509 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,75,552.50
GST (18%): ₹ 31,599.45
Total Price: ₹ 2,07,151.95
Antigen
Amphiregulin
Classification
Polyclonal
Conjugate
Biotin
Gene
AREG
Gene Alias
amphiregulin; amphiregulin B; AR; AREG; AREGB; colorectum cell-derived growth factor; CRDGF; Mcub; MGC13647; schwannoma-derived growth factor; SDGF
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
374
Content And Storage
-20°C
Isotype
IgG
Applications
Western Blot, ELISA
Concentration
0.1-1.0 mg/mL
Formulation
PBS with no preservative
Gene Accession No.
P15514
Gene Symbols
AREG
Immunogen
E.coli-derived Recombinant Human Amphiregulin
Quantity
500μg
Primary or Secondary
Primary
Target Species
Human
Form
Lyophilized
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Description
- AA Sequence of recombinant protein: SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK
- Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Amphiregulin
- Anti-Human Amphiregulin-specific antibody was purified by affinity chromatography and then biotinylated
- Sandwich ELISA: To detect Human Amphiregulin by sandwich ELISA (using 100 μL/well), a concentration of 0.25-1.0 μg/mL of this antibody is required
- This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Amphiregulin (500-P322) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human Amphiregulin
- Western Blot: To detect Human Amphiregulin by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/mL
- When used in conjunction with compatible development reagents, the detection limit for Recombinant Human Amphiregulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
- 500-P322BT-1MG will be provided as 2 x 500 μg Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF
- The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor
- Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR
- These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin
- All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds
- AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta
- AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.