3G11 Sialoganglioside Antigen Monoclonal Antibody (eBio3G11 (SM3G11, 3G11)), eFluor™ 660, eBioscience™, Invitrogen™

Description

• The eBio3G11 MAb reacts with the mouse 3G11antigen
• The 3G11 antibody was isolated as an autoantibody against the 3G11 sialylated carbohydrate antigen, which was found to be expressed by thymoyctes and peripheral T cells, but not by B cells, myeloid cells or erythrocytes
• 3G11 is expressed at higher levels on CD4+ T cells than on CD8+ T cells, however expression on both of these subsets decreases with advancing age
• In thymus, 3G11 is expressed on approximately 20% of total thymocytes
• 3G11 has also been used as a marker to distinguish naive and memory T cells, as 3G11 is irreversibly down-regulated upon activation of CD4+ T cells
• The 3G11 antibody is able to block binding of antibodies to CD3 epsilon, suggesting close proximity of these two proteins
• Recently, it has been demonstrated that loss of 3G11 expression in experimental autoimmune encephalomyelitis (EAE) is associated with an anergic, regulatory T phenotype
• Purified 3G11- T cells were able to suppress proliferation and cytokine production of 3G11+ T cells, and part of this effect was due to the secretion of IL-10
• The 3G11 sialylated carbohydrate antigen was found to be expressed by thymoyctes and peripheral T cells, but not by B cells, myeloid cells or erythrocytes
• 3G11 is expressed at higher levels on CD4+ T cells than on CD8+ T cells, however expression on both of these subsets decreases with advancing age
• In thymus, 3G11 is expressed on approximately 20% of total thymocytes
• 3G11 has also been used as a marker to distinguish naive and memory T cells, as 3G11 is irreversibly down-regulated upon activation of CD4+ T cells
• 3G11 antibodies can be used to block binding of antibodies to CD3 epsilon, suggesting close proximity of these two proteins
• Recently, it has been demonstrated that loss of 3G11 expression in experimental autoimmune encephalomyelitis (EAE) is associated with an anergic, regulatory T phenotype
• Purified 3G11- T cells were able to suppress proliferation and cytokine production of 3G11+ T cells, and part of this effect was due to the secretion of IL-10.