50-786-593
His Tag Mouse anti-Tag, Clone: OTI2B5, lyophilized, TrueMAB™
Manufacturer: OriGene
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-786-593-Each-of-1 | In Stock | ₹ 38,585.95 |
50-786-593 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,585.95
GST (18%): ₹ 6,945.471
Total Price: ₹ 45,531.421
Antigen
His Tag
Classification
Monoclonal
Concentration
4 mg/mL
Formulation
PBS with 8% trehalose and no preservative
Host Species
Mouse
Purification Method
Protein G
Regulatory Status
RUO
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Lyophilized
Target Molecule
Tag
Applications
Western Blot
Clone
OTI2B5
Conjugate
Unconjugated
Gene Alias
ANTI HIS, ANTI HISTIDINE, ANTIHIS, anti-His, his tag antibody, Anti-His Antibody, his antibody, anti his tag, anti-his tag
Immunogen
Anti-His monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH
Quantity
400 μg
Primary or Secondary
Primary
Product Type
Antibody
Isotype
IgG1
Related Products
Description
- Synthetic peptide YPYDVPDYA conjugated to KLH 400 μg lyophilized powder
- The lyophilized antibody can be reconstituted with 100 μL of ddH2O
- The concentration after reconstitution will be 4 μg/μL
- The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH)
- The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombit proteins to allow isolation or purification by immobilized metal affinity chromatography
- Epitope tagging is a technique in which a known epitope is fused to a recombit protein using genetic engineering
- By choosing a particular epitope and recombit protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available
- His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombit proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins
- For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent.