IL-17FF (homodimer) Monoclonal Antibody (WU24A4P), eFluor™ 660, eBioscience™, Invitrogen™

Description

• The WU24A4P MAb reacts with human interleukin (IL)-17F homodimer (IL-17FF)
• This antibody specifically reacts with this homodimer and has no reactivity for the IL-17AF heterodimer
• IL-17FF is a 34 kDa member of the IL-17 family, which includes IL-17A, B, C, D, and E (IL-25)
• Within this family, IL-17A and IL-17F are produced preferentially by T helper 17 (Th17) cells, with other members produced more widely
• IL-17F and IL-17A have many overlapping functions, but knockout studies have shown each to have independent functions as well
• In vitro cultured Th17 cells have been shown to express IL-17FF and IL-17AA homodimers and IL-17AF heterodimers depending on the culture conditions and their differentiation state
• Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis
• While other clones with specificity for IL-17F, such as SHLR17, react with the IL-17F monomer in the context both IL-17FF and IL-17AF dimers, clone WU24A4P reacts specifically with IL-17FF homodimer and does not detect IL-17AF heterodimer
• Interleukin 17F is a cytokine belonging to the IL-17 family that is produced by inflammatory cells such as activated T cells, mast cells, and basophils
• IL-17F is involved in allergic airway inflammation, and can induce several cytokines, chemokines, and adhesion molecules in bronchial epithelial cells, vein endothelial cells, fibroblasts, and eosinophils
• IL-17F may be secreted as a homodimer, or a heterodimer with IL17A
• It acts by binding to the type I receptor, IL-17R, aiding recruitment of monocytes and neutrophils at the site of inflamation by increasing chemokine production.