APE Antibody - BSA Free, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 64 publications
Manufacturer: Fischer Scientific
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Antigen
APE
Dilution
Western Blot 1:1000, Simple Western 1:12.5, Flow Cytometry, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 7 ug/ml, Immunohistochemistry-Paraffin 1:100, Immunohistochemistry-Frozen 1:100, Immunohistochemistry Free-Floating reported in scientific literature (PMID 17332344), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Block/Neutralize
Classification
Polyclonal
Host Species
Rabbit
Research Discipline
Autophagy, Base Excision Repair, Cancer, Core ESC Like Genes, DNA Repair, Stem Cell Markers
Formulation
Tris-Glycine and 0.15M NaCl with 0.05% Sodium Azide
Gene Alias
AP endonuclease 1, APE, APE1, APE-1, APEAPEX nuclease (multifunctional DNA repair enzyme), APENAP endonuclease class I, APEX deoxyribonuclease (apurinic or apyrimidinic), APEX nuclease, APEX nuclease (multifunctional DNA repair enzyme) 1, Apurinic-apyrimidinic endonuclease 1, APXAP lyase, DNA-(apurinic or apyrimidinic site) lyase, EC 3.1, EC 4.2.99.18, HAP1apurinic/apyrimidinic (abasic) endonuclease, multifunctional DNA repair enzyme, protein REF-1, redox factor 1, Redox factor-1, REF-1, REF1 apurinic/apyrimidinic exonuclease
Gene Symbols
APEX1
Isotype
IgG
Purification Method
Affinity Purified
Test Specificity
Human, mouse, primate and rat. Rabbit reactivity reported in the scientific literature (PMID: 15276530).
Concentration
0.5 mg/mL
Applications
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Free Floating), Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization
Conjugate
Unconjugated
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Primate, Rabbit
Gene Accession No.
P27695
Gene ID (Entrez)
328
Immunogen
Affinity purified human APE1 [UniProt# P27695]
Primary or Secondary
Primary
Content And Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Molecular Weight of Antigen
37 kDa
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Description
- APE Polyclonal specifically detects APE in Human, Mouse, Rat, Primate, Rabbit samples
- It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Block/Neutralize.