LC3B Antibody - BSA Free, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 1218 publications
Manufacturer: Fischer Scientific
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Antigen
LC3B
Dilution
Western Blot 0.5 - 2.0 ug/mL, Simple Western 1:50, Flow Cytometry, ELISA, Immunohistochemistry 1:200 - 1:400, Immunocytochemistry/Immunofluorescence 1:200, Immunoprecipitation 20 ug/500 ug of protein, Immunohistochemistry-Paraffin 1:200 - 1:400, Immunohistochemistry-Frozen, Immunoblotting, Proximity Ligation Assay, SDS-Page, Chromatin Immunoprecipitation (ChIP), Knockout Validated, KnockDown Validated
Classification
Polyclonal
Host Species
Rabbit
Research Discipline
Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Hypoxia, Innate Immunity, Lipid and Metabolism, Macroautophagy, Mitophagy, Neuroscience
Formulation
PBS with 0.02% Sodium Azide
Gene Alias
anti-LC3B, ATG8F, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, lc3b autophagy marker, LC3B ihc, LC3B immunohistochemistry, LC3B immunoprecipitation, LC3B western blot, lc3-i, ii, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, map1lc3b, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta, microtubule associated protein 3 b, microtubule associated protein 3 beta, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B
Gene Symbols
MAP1LC3B
Isotype
IgG
Purification Method
Affinity Purified
Test Specificity
Human, rat, mouse, zebrafish, bacteria, hamster, and porcine. Bovine reactivity reported in scientific literature (PMID: 24895572) Primate reactivity reported in scientific literature (PMID: 25142602) The mouse detection has been reported to be weaker than the human. Immunogen sequence has 84% homology to Xenopus.
Concentration
1.0 mg/mL
Applications
ChIP Assay, ELISA, Flow Cytometry, Immunoassay, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In situ PLA, KnockDown
Conjugate
Unconjugated
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Porcine, Alligator, Avian, Bacteria, Bovine, Canine, Chicken, Guinea Pig, Hamster, Invertebrate, Monkey, Primate, Rabbit, Golden Syrian Hamster, Zebrafish
Gene Accession No.
Q9GZQ8
Gene ID (Entrez)
81631
Immunogen
Polyclonal LC3B Antibody was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100). [UniProt# Q9GZQ8]
Primary or Secondary
Primary
Content And Storage
Store at -20°C.
Molecular Weight of Antigen
14.688 kDa
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Description
- LC3B Polyclonal specifically detects LC3B in Human, Mouse, Rat, Porcine, Alligator, Avian, Bacteria, Bovine, Canine, Chicken, Chinese Hamster, Guinea Pig, Hamster, Invertebrate, Monkey, Primate, Rabbit, Golden Syrian Hamster, Zebrafish samples
- It is validated for Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Proximity Ligation Assay, SDS-Page, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Knockdown Validated.