5071982

HIF-1 alpha Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 175 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

HIF-1 alpha

Dilution

Western Blot 1:500 - 1:1000, Simple Western 1:100, ELISA, Immunohistochemistry 1:100 - 1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:1000, Immunohistochemistry-Paraffin 1:100 - 1:500, Immunohistochemistry-Frozen 1:100 - 1:500, Immunoblotting, Gel Super Shift Assays 1:1 - 1:100, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Chromatin Immunoprecipitation Sequencing reported in scientific literature (PMID 34277635), Dual RNAscope ISH-IHC

Classification

Polyclonal

Host Species

Rabbit

Research Discipline

Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators

Formulation

PBS with 0.02% Sodium Azide

Gene Alias

AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp

Gene Symbols

HIF1A

Isotype

IgG

Purification Method

Affinity Purified

Test Specificity

Human, monkey, mouse, rat and Xenopus. Canine reactivity reported in scientific literature (PMID: 24140705) Mouse results are mixed.

Concentration

1.0 mg/mL

Applications

ChIP Assay, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot, Gel Shift, ChIP Assay, Immunoassay

Conjugate

Unconjugated

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Primate, Xenopus species, Zebrafish

Gene Accession No.

Q61221, O35800

Gene ID (Entrez)

3091

Immunogen

This HIF-1 alpha Antibody was developed against a fusion protein made to an internal sequence of human HIF-1 alpha (containing amino acids 432 - 528) [Uniprot# Q16665].

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen

93 kDa

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Description

  • HIF-1 alpha Polyclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Primate, Xenopus, Zebrafish samples
  • It is validated for Western Blot, Simple Western, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Gel Super Shift Assays, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Chromatin Immunoprecipitation Sequencing, Dual RNAscope ISH-IHC.