Cytokeratin 5/8 Antibody (SPM268), Novus Biologicals™

Cytokeratin 5/8

0.2mg/mL

Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Unconjugated

Mouse

Human, Mouse, Rat, Porcine, Canine, Ferret, Hamster, Primate, Sheep, Chicken (Negative), Xenopus (Negative)

P13647, P05787

3852

Cytoskeletal preparation from HeLa cells

Primary

Store at 4C.

SPM268

Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Monoclonal

Purified

RUO

10mM PBS (pH 7.4) and 0.05% BSA with 0.05% Sodium Azide

cell proliferation-inducing protein 46, CK18, CK5, CYK18, DDD, K18, K1CO, K5, Keratin, Keratin 18, Keratin 5, KRT18, krt5, KRT5A, KRTB, type I cytoskeletal 18, type II cytoskeletal 5

KRT5

IgG1 κ

Protein A or G purified

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.