Cytokeratin 5/8 Antibody (C-50) - Azide and BSA Free, Novus Biologicals™
Manufacturer: Fischer Scientific
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Antigen
Cytokeratin 5/8
Classification
Monoclonal
Concentration
1.0 mg/mL
Dilution
Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias
cell proliferation-inducing protein 46, CK18, CK5, CYK18, DDD, K18, K1CO, K5, Keratin, Keratin 18, Keratin 5, KRT18, krt5, KRT5A, KRTB, type I cytoskeletal 18, type II cytoskeletal 5
Host Species
Mouse
Purification Method
Protein A or G purified
Regulatory Status
RUO
Gene ID (Entrez)
3852
Target Species
Human, Mouse, Rat, Porcine, Canine, Ferret, Hamster, Primate, Sheep, Chicken (Negative), Xenopus (Negative)
Form
Purified
Applications
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone
C-50
Conjugate
Unconjugated
Formulation
PBS with No Preservative
Gene Symbols
KRT5
Immunogen
Cytoskeletal preparation from HeLa cells
Quantity
0.2 mg
Primary or Secondary
Primary
Test Specificity
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype
IgG1 κ
Related Products
Description
- Cytokeratin 5/8 Monoclonal specifically detects Cytokeratin 5/8 in Human, Mouse, Rat, Porcine, Bovine, Canine, Hamster, Ferret, Sheep, Chicken (Negative), Xenopus (Negative) samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.