alpha-Fetoprotein/AFP Antibody (C2 + C3 + MBS-12), Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
alpha-Fetoprotein/AFP
Concentration
0.2mg/mL
Applications
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cancer, Cancer Stem Cells, GPCR, Stem Cell Markers, Tumor Biomarkers
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias
Alpha-1-fetoprotein, Alpha-fetoglobulin, alpha-fetoprotein, FETA, HP, HPAFP
Gene Symbols
AFP
Isotype
IgG
Purification Method
Protein A or G purified
Test Specificity
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
Clone
C2 + C3 + MBS-12
Dilution
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 1 - 2 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Accession No.
P02771
Gene ID (Entrez)
174
Immunogen
Recombinant full-length human Alpha fetoprotein
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Molecular Weight of Antigen
70 kDa
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Description
- Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence alpha-Fetoprotein/AFP Monoclonal specifically detects alpha-Fetoprotein/AFP in Human samples
- It is validated for Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.