Glypican 3 Antibody (1G12 + GPC3/863), Novus Biologicals™

Glypican 3

0.2mg/mL

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Unconjugated

Mouse

Human

P51654, P51654

2719

Recombinant fragment containing amino acids 511-580 of human glypican-3 (1G12); Recombinant full-length human GPC3 protein (GPC3/863)

Primary

Store at 4C.

67 kDa

1G12 + GPC3/863

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Monoclonal

Purified

RUO

1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide

DGSX, glypican 3, glypican proteoglycan 3, glypican-3, GTR2-2, heparan sulphate proteoglycan, Intestinal protein OCI-5, MXR7, OCI5, OCI-5, secreted glypican-3, SGB, SGBS, SGBS1SDYS

GPC3

IgG

Protein A or G purified

Glypican-3 (GPC3) is an integral membrane protein that is mutated in the Simpson-Golabi-Behmel syndrome (SGBS). SGBS is characterized by pre- and post-natal overgrowth and is a recessive X-linked condition. GPC3 may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 ex