TNF-alpha Antibody (J2D10), Novus Biologicals™

TNF-alpha

0.2 mg/mL

Flow Cytometry, SDS-Page, Immunofluorescence

Unconjugated

Mouse

Adaptive Immunity, Asthma, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha

Recombinant human TNF-alpha

Primary

Store at 4C.

17 kDa

J2D10

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml

Monoclonal

Purified

RUO

Human, Rat, Mouse (Negative)

P01375

7124

IgG1 κ

Protein A or G purified

This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF