Gata-3 Monoclonal Antibody (TWAJ), Alexa Fluor™ 700, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 56-996-642-Each-of-1 | In Stock | ₹ 38,715.00 |
56-996-642 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,715.00
GST (18%): ₹ 6,968.70
Total Price: ₹ 45,683.70
Antigen
Gata-3
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.
P23771, P23772
Gene Symbols
GATA3
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
14462, 2625, 713840, 733631
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
TWAJ
Conjugate
Alexa Fluor 700
Gene
GATA3
Gene Alias
EGK_19445, GATA binding factor-3, GATA binding protein 3, GATA binding protein-3, GATA3, Gata-3, GATA-binding factor 3, GATA-binding protein 3, HDR, HDRS, jal, MGC2346, MGC5199, MGC5445, Trans-acting T-cell-specific transcription factor GATA-3, Transacting T-cell-specific transcription factor GATA-3
Host Species
Rat
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human, Mouse, Porcine, Rhesus Monkey
Product Type
Antibody
Isotype
IgG2b κ
Related Products
Description
- Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors
- Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation
- Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney
- During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal
- The Gata-3 is also essential for T cell commitment and survival
- In the thymus, expression is found mainly on the CD4 single positive cells
- During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation
- Alternative splice variants have been reported especially in the MCF7 cell line
- The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein
- Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set (Product No
- 00-5523-00)
- Crossreactivity in rhesus monkeys has been published
- Applications Reported: This TWAJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This TWAJ antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product No
- The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins
- Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes
- GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain
- GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages
- GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers
- Labastie et al
- (1994) cloned the human gene and the 5-prime end of the mouse gene
- The human gene comprises 6 exons distributed over 17 kb of DNA
- Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.