CD63 Monoclonal Antibody (H5C6), PE-eFluor™ 610, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each | 61-063-942-Each | In Stock | ₹ 29,427.85 |
61-063-942 - Each
In Stock
Quantity
1
Base Price: ₹ 29,427.85
GST (18%): ₹ 5,297.013
Total Price: ₹ 34,724.863
Antigen
CD63
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.
P08962
Gene Symbols
CD63
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
967
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
H5C6
Conjugate
PE-eFluor 610
Gene
CD63
Gene Alias
C75951, CD 63, Cd63, CD63 antigen, CD63 antigen (melanoma 1 antigen), Cd63 molecule, CD63 protein, granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, lysosome-associated membrane glycoprotein 3, mast cell antigen AD1, ME491, melanoma 1 antigen, melanoma-associated antigen ME491, melanoma-associated antigen MLA1, MLA1, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, TSPAN30, tspan-30
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1 κ
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Description
- Description: This H5C6 monoclonal antibody reacts with human CD63, a type III member of the tetraspanin family of transmembrane proteins
- CD63 is expressed intracellularly on lysosomes, endosomes, and granules of resting platelets and basophils
- However, cell surface expression of CD63 can be detected on activated basophils and platelets, monocytes, macrophages, and granulocytes
- This receptor is also expressed on endothelial cells, fibroblasts, and smooth muscle cells
- Studies have demonstrated that CD63 associates with integrins (VLA-3 and VLA-6) and TIMP-1 to mediate the allergic response
- Applications Reported: This H5C6 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This H5C6 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (1.0 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm
- We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red)
- Please make sure that your instrument is capable of detecting this fluorochrome
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types
- CD63 is also present in the plasma membrane, usually following cell activation
- Hence, CD63 has become a widely used basophil activation marker
- In mast cells, however, CD63 exposition does not need their activation
- CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels
- CD63 also serves as a T-cell costimulation molecule
- Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas
- CD63 is expressed on activated platelets, and is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation
- CD63 is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells
- CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40
- Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.