CD69 Monoclonal Antibody (FN50), PE-eFluor™ 610, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 61-069-942-Each-of-1 | In Stock | ₹ 32,040.00 |
61-069-942 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 32,040.00
GST (18%): ₹ 5,767.20
Total Price: ₹ 37,807.20
Antigen
CD69
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.
Q07108
Gene Symbols
CD69
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
969
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
FN50
Conjugate
PE-eFluor 610
Gene
CD69
Gene Alias
5830438K24Rik, Activation inducer molecule, activation inducer molecule (AIM/CD69), AI452015, AIM, BL-AC/P26, CD69, CD69 antigen, CD69 antigen (p60, early T-cell activation antigen), CD69 molecule, CLEC2C, C-type lectin domain family 2 member C, C-type lectin domain family 2, member C, EA1, early activation antigen CD69, early lymphocyte activation antigen, early T-cell activation antigen p60, GP32/28, leukocyte surface antigen Leu-23, MLR-3, VEA, Very Early Activation Antigen
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- Description: The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA)
- CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer
- CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets
- Applications Reported: This FN50 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This FN50 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (0.25 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm
- We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red)
- Please make sure that your instrument is capable of detecting this fluorochrome
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (Product No
- 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No
- 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
- CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions
- CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets
- In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction
- Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation
- Furthermore, the protein may act to transmit signals in natural killer cells and platelets
- Alternative splicing results in multiple transcript variants of CD69
- Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.