HLA-A3 Monoclonal Antibody (GAP.A3), Super Bright™ 600, eBioscience™, Invitrogen™
Manufacturer: eBioscience
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 63-575-442-Each-of-1 | In Stock | ₹ 35,422.00 |
63-575-442 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 35,422.00
GST (18%): ₹ 6,375.96
Total Price: ₹ 41,797.96
Antigen
HLA-A3
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
P04439
Gene Symbols
HLA-A
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
3105
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
GAP.A3
Conjugate
Super Bright 600
Gene
HLA-A
Gene Alias
A29 protein; antigen presenting molecule; Aw-33; Aw-74; HLA A; HLA class I antigen; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-30 alpha chain; HLA class I histocompatibility antigen, A-33 alpha chain; HLA class I histocompatibility antigen, A-74 alpha chain; HLA locus; HLAA; HLA-A; HLA-A locus alpha 2 domain; HLA-A null; HLA-A11; HLA-A33; HLA-Aw33.1; HLA-DQB1; HLA-DRB1; Human leukocyte antigen A; leukocyte antigen class I-A; major histocompatibility complex, class I, A; MCH class I antigen; MHC class 1 antigen; MHC class I anti; MHC Class I antigen; MHC class I antigen A*30; MHC class I antigen A*33; MHC class I antigen A*74; MHC class I antigen heavy chain; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-A33; MHC class I antigen null protein; MHC class I antigene; MHC class II antigen; MHC classI antigen; truncated MHC class I antigen
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG2a κ
Related Products
Description
- Description: The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, which is a member of the HLA-A family
- HLA-A3 exists as two subtypes, HLA-A3.1 and HLA-A3.2, which differ at amino acid positions 152 and 156
- MHC class I molecules are expressed on all nucleated cells
- These molecules are highly polymorphic, cell surface glycoproteins that non-covalently associate with B2 microglobulin
- MHC Class I molecules present intracellular peptides on the cell surface and nonnative peptides are recognized by circulating cytotoxic T lymphocytes
- HLA-A3 is expressed by 15-25% of cancer patients and has been demonstrated to bind the tumor-associated antigen human telomerase reverse transcriptase (hTERT)
- Applications Reported: This GAP.A3 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This GAP.A3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
- This can be used at 5 μL (0.5 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
- We recommend using a 610/20 bandpass filter
- HLA-A belongs to the HLA class I heavy chain paralogues
- This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin)
- The heavy chain is anchored in the membrane
- Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen
- They are expressed in nearly all cells
- The heavy chain is approximately 45 kDa and its gene contains 8 exons
- Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail
- Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule
- Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation
- Hundreds of HLA-A alleles have been described
- HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.