CD2 Monoclonal Antibody (RPA-2.10), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex
• Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex
• CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells
• Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized
• 145-2C11 is commonly used as a phenotypic marker for mouse T cells
• Applications Reported: This 145-2C11 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 145-2C11 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm
• We recommend using a 660/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• CD2 (LFA-2) is a monomeric surface antigen (MW range 45-58 kDa) of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells
• CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes and it is also found on some natural killer cells, but not on B lymphocytes
• Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it
• The interaction between CD2 and CD58 stabilizes adhesion between T cells and antigen presenting or target cells
• Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion
• Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58
• Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion
• CD2 deficiency reduces intestinal inflammation and helps to control infection
• Diseases associated with CD2 dysfunction include penis squamous cell carcinoma and immune deficiency due to the absence of the thymus.