Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright™ 645, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 64-598-182-Each-of-1 | In Stock | ₹ 34,532.00 |
64-598-182 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 34,532.00
GST (18%): ₹ 6,215.76
Total Price: ₹ 40,747.76
Antigen
Ly-6A/E (Sca-1)
Classification
Monoclonal
Concentration
0.2 mg/mL
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
P05533, Q64253
Gene Symbols
Ly6a, Ly6e
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
110454, 17069
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
D7
Conjugate
Super Bright 645
Gene
Ly6e
Gene Alias
9804; locus A/E; Locus AE; Ly6; Ly67; Ly6a; Ly-6A.2; Ly-6A.2/Ly-6E.1; Ly6A/E; Ly-6A/E; ly6a-e; Ly6e; Ly-6E; Ly-6E.1; lymphocyte antigen 6 complex; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6 complex, locus E; lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; RIG-E; Sca1; Sca-1; Sca-2; stem cell antigen 1; Stem cell antigen 2; TAP; T-cell-activating protein; thymic shared antigen 1; Tsa1; TSA-1
Host Species
Rat
Quantity
100μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2a κ
Related Products
Description
- Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins
- D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells
- Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others
- Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype
- The Ly-6 family is involved in regulation and function of T cell activation
- Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset
- Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm
- We recommend using a 660/20 bandpass filter
- Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family
- It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice
- Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC
- Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen
- Sca-1 may be involved in the regulation of B and T cell activation.