CD284 (TLR4) Monoclonal Antibody (HTA125), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family
• A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response
• ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases
• ICOS may be involved in the development of Th2 cells
• Applications Reported: This 7E.17G9 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 7E.17G9 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm
• We recommend using a 660/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity
• TLRs are highly conserved from Drosophila to humans and share structural and functional similarities
• This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes
• Mammalian cells respond to LPS by activating TLR4
• TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria
• TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity
• The various TLRs exhibit different patterns of expression
• Mutations in the TLR4 gene have been associated with differences in LPS responsiveness
• Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain
• TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta
• Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide
• Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.