IRF7 Monoclonal Antibody (MNGPKL), PE, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody MNGPKL reacts with mouse interferon regulatory factor 7 (IRF7), a 54 kDa transcription factor essential for induction of the antiviral innate immune response and IFN alpha production
• B cells, plasmacytoid dendritic cells (pDC), and monocytes constitutively express IRF7 in the cytoplasm until activation by viral infection, double-stranded RNA or Toll-like receptor (TLR) signaling
• CpG ODNs and single-stranded RNA, recognized by CD289 (TLR9) and TLR7, respectively, trigger IRF7 activation and upregulation
• This activation promotes type I IFN response to disrupt pathogen and viral infection
• These events cause IRF7 phosphorylation and translocation to the nucleus where it forms a homodimer to affect transcription
• Applications Reported: This MNGPKL antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This MNGPKL antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat
• 88-8824) and protocol
• Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms
• During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes
• IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes
• IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling
• There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.