CD252 (OX40 Ligand) Monoclonal Antibody (RM134L), PE, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD252 (OX40 Ligand)
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
Ath1; Ath-1; atherosclerosis 1; CD134 ligand; CD134L; CD252; glycoprotein Gp34; GP34; OX40 antigen ligand; OX40 ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1 ligand; TNFSF4; TNLG2B; tumor necrosis factor (ligand) superfamily member 4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand 2B; tumor necrosis factor ligand superfamily member 4; tumor necrosis factor superfamily member 4; TXGP1; Txgp1l
Gene Symbols
Tnfsf4
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Tnfsf4
Clone
RM134L
Applications
Flow Cytometry
Conjugate
PE
Host Species
Rat
Target Species
Mouse
Gene Accession No.
P43488
Gene ID (Entrez)
22164
Isotype
IgG2b κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The RM134L monoclonal antibody reacts with mouse CD252 also known as OX-40 Ligand, a member of the TNF superfamily
- OX-40L is induced on mouse splenic B cells stimulated with a combination of anti-IgM and anti-CD40
- Neither resting nor activated mouse T cells express OX-40L
- The interaction of OX-40 (CD134) with OX-40L has been implicated in T cell-dependent humoral response
- RM134L inhibits the costimulatory activity of mouse OX-40L transfected cells for anti-CD3-stimulated T cell proliferation
- Applications Reported: This RM134L antibody has been reported for use in flow cytometric analysis
- Applications Tested: The RM134L antibody has been tested by flow cytometric analysis of activated mouse splenocytes and can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
- Filtration: 0.2 μm post-manufacturing filtered
- The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein
- It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells
- TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells
- TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells
- It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma
- TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.