CD261 (DR4) Monoclonal Antibody (DJR1), PE, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
CD261 (DR4)
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias
APO2; CD261; cytotoxic TRAIL receptor; death receptor 4; DR4; MGC9365; sCD261; soluble CD261; soluble TRAIL; sTRAIL; TNF receptor superfamily member 10a; TNF-related apoptosis-inducing ligand receptor 1; TNFRSF10A; TRAIL R1; TRAIL receptor 1; TRAILR1; TRAIL-R1; TRAILR-1; tumor necrosis factor receptor superfamily member 10A; tumor necrosis factor receptor superfamily member 10a variant 2; tumor necrosis factor receptor superfamily, member 10a
Gene Symbols
TNFRSF10A
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
TNFRSF10A
Clone
DJR1
Applications
Flow Cytometry
Conjugate
PE
Host Species
Mouse
Target Species
Human
Gene Accession No.
O00220
Gene ID (Entrez)
8797
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The DJR1 monoclonal antibody reacts with human DR4, also known as TRAIL-R1
- DR4 binds to TRAIL, activates NF-kappaB, and induces TRAIL-mediated apoptosis
- DR4 is expressed by a variety of human tumor cells and at very low level on human leukocytes
- Expression has also been found inside the cell on some cell lines (Zhang et al)
- This has been confirmed with intracellular staining of Jurkat cell line
- Please contact tech@ebioscience.com for more information
- Applications Reported: The DJR1 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This DJR1 antibody has been pre-titrated and tested by flow cytometric analysis of human DR4-transfected cells
- This can be used at 5 μL (0.5 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
- Filtration: 0.2 μm post-manufacturing filtered
- TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1
- The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family
- DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues
- Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms
- Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).