IL-4 Monoclonal Antibody (11B11), PE, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
IL-4
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
B cell growth factor 1; B_cell stimulatory factor 1; B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; cytokine; H-IL-4; IGG1 induction factor; IL2; IL4; Il-4; Il4e12; ILN; Interleukin; interleukin 4; interleukin 4 variant 2; interleukin 4 variant IL-4delta2; interleukin 4 variant IL-4delta3; interleukin 4 variant IL-4int2A; interleukin 4 variant IL-4int3A; interleukin 4 variant IL-4int3B; Interleukin4; interleukin-4; interleukin-4 precursor; lymphocyte stimulatory factor 1; MGC79402; M-IL-4; Pitrakinra
Gene Symbols
Il4
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Il4
Clone
11B11
Applications
Flow Cytometry
Conjugate
PE
Host Species
Rat
Target Species
Mouse
Gene Accession No.
P07750
Gene ID (Entrez)
16189
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
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Description
- Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine
- The 11B11 antibody is a neutralizing antibody
- Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells
- IL-4 is species-specific and promotes proliferation and differentiation of B cells
- IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23
- Applications Reported: This 11B11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This 11B11 antibody is offered in 2 formats: - μg size: has been tested by intracellular staining of Th2 polarized and stimulated mouse splenocytes
- This can be used at less than or equal to 0.125 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- - test size: has been pre-titrated and tested by intracellular staining of Th2 polarized and stimulated mouse splenocytes
- This can be used at 5 μL (0.06 μg) per test
- Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor
- IL-4 has been found to have the broadest range of actions
- The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13
- STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4
- The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q
- In particular, IL-4 is located in close proximity to IL13 on the chromosome
- Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome
- IL-4 delta 2 is a known splice variant of IL-4.