IL-4 Monoclonal Antibody (11B11), eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
IL-4
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
B cell growth factor 1; B_cell stimulatory factor 1; B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; cytokine; H-IL-4; IGG1 induction factor; IL2; IL4; Il-4; Il4e12; ILN; Interleukin; interleukin 4; interleukin 4 variant 2; interleukin 4 variant IL-4delta2; interleukin 4 variant IL-4delta3; interleukin 4 variant IL-4int2A; interleukin 4 variant IL-4int3A; interleukin 4 variant IL-4int3B; Interleukin4; interleukin-4; interleukin-4 precursor; lymphocyte stimulatory factor 1; MGC79402; M-IL-4; Pitrakinra
Gene Symbols
Il4
Primary or Secondary
Primary
Content And Storage
4° C
Gene
Il4
Clone
11B11
Applications
Immunostaining
Conjugate
Unconjugated
Host Species
Rat
Target Species
Mouse
Gene Accession No.
P07750
Gene ID (Entrez)
16189
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine
- The 11B11 antibody is a neutralizing antibody
- Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells
- IL-4 is species-specific and promotes proliferation and differentiation of B cells
- IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23
- Applications Reported: The 11B11 antibody has been reported for use in ELISA capture, ELISPOT capture, and neutralization of IL-4 bioactivity
- Applications Tested: The 11B11 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-4 (IL-4) in combination with the biotin BVD6-24G2 (cat
- 13-7042) antibody for detection and recombinant mouse IL-4 (cat
- 14-8041) as the standard
- A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL
- A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate
- Purity: Greater than 90%, as determined by SDS-PAGE
- Aggregation: Less than 10%, as determined by HPLC
- Filtration: 0.2 μm post-manufacturing filtered
- Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor
- IL-4 has been found to have the broadest range of actions
- The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13
- STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4
- The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q
- In particular, IL-4 is located in close proximity to IL13 on the chromosome
- Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome
- IL-4 delta 2 is a known splice variant of IL-4.