GM-CSF Monoclonal Antibody (MP1-22E9), PE, eBioscience™, Invitrogen™

Description

• Description: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF)
• The MP1-22E9 antibody is a neutralizing antibody
• Mouse GM-CSF is a 14 kDa factor produced mainly by activated T cells and macrophages
• Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-alpha, IL-2, IL-1, and IFN-gamma
• GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells
• GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix
• Non-glycosylated GM-CSF is biologically active
• Applications Reported: MP1-22E9 has been reported for use in intracellular flow cytometric analysis
• Applications Tested: This MP1-22E9 antibody is tested by intracellular staining and flow cytometric analysis of cultured mouse splencoytes
• It is offered in 2 formats: - μg size: has been tested bu intracellular staining and flow cytometric analysis of cultured mouse splencoytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes
• The active form of the protein exists as a homodimer in the extracellular space
• This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia
• Other genes in the cluster include interleukins 4, 5, and 13
• The gene is involved in promoting tissue inflammation
• Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome
• Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.