CD5 Monoclonal Antibody (53-7.3), FITC, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD5
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
alanyl (membrane) aminopeptidase; alanyl aminopeptidase; alanyl aminopeptidase, membrane; aminopeptidase M; Aminopeptidase N; ANPEP; AP-M; APN; AP-N; CD antigen CD5; CD13; CD28; CD28 antigen (Tp44); CD28 molecule; Cd5; CD5 antigen; CD5 antigen (p56 62); CD5 antigen (p56-62); CD5 antigen p56-62; CD5 molecule; CD5 protein; CD74 antigen, invariant polypeptide of major; cell surface protein; costimulatory molecule B7 receptor CD28; fCD5; GP150; hAPN; LAP1; LEU1; Ly-1; Ly12; Ly-12; LyA; Ly-A; Lymphocyte antigen 1; Lymphocyte antigen CD5; lymphocyte antigen T1/Leu-1; Lyt-1; membrane alanyl aminopeptidase; microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; P150; Pan T cell; PEPN; T1; T-cell costimulatory molecule CD28; T-cell surface glycoprotein CD5; T-cell-specific surface glycoprotein CD28; unnamed protein product
Gene Symbols
Cd5
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Cd5
Clone
53-7.3
Applications
Flow Cytometry
Conjugate
FITC
Host Species
Rat
Target Species
Mouse
Gene Accession No.
P13379
Gene ID (Entrez)
12507
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells
- The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells
- CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction
- Applications Reported: The 53-7.3 antibody has been reported for use in flow cytometric analysis
- Applications Tested: The 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
- Filtration: 0.2 μm post-manufacturing filtered
- CD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein
- CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas
- CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation
- CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes
- In lymph nodes, the main reactivity is observed in T cell areas
- CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells
- Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome.