7068798
SIGLEC H Monoclonal Antibody (eBio440c), PE, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
SIGLEC H
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
6430529G09Rik; sialic acid binding Ig-like lectin H; Siglec; Siglech; Siglec-H; SIGLEC-like protein
Gene Symbols
Siglech
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Siglech
Clone
eBio440c
Applications
Flow Cytometry
Conjugate
PE
Host Species
Rat
Target Species
Mouse
Gene Accession No.
0
Gene ID (Entrez)
233274
Isotype
IgG2b κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse
- Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses
- Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif)
- To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction
- The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG)
- It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6
- Applications Reported: This eBio440c antibody has been reported for use in flow cytometric analysis
- Applications Tested: This eBio440c antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 0.125 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse
- Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses
- Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif)
- To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction
- The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG)
- It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.