CD150 Monoclonal Antibody (9D1), PE, eBioscience™, Invitrogen™

Description

• Description: The 9D1 monoclonal antibody reacts with mouse CD150, an ∽70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM)
• CD150 is expressed by T (especially TH1) and B cells and its expression is rapidly upregulated on these cells upon activation
• Immature thymocytes and dendritic cells also express this antigen
• Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response
• Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell
• 9D1 is reported to be an activating antibody
• Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers, such as CD150+CD244-CD48-
• For this application we recommend the use of antibody clone mShad150 (cat
• 12-1502)
• Applications Reported: This 9D1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 9D1 antibody has been tested by flow cytometric analysis of mouse thymic and splenic cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily, and is involved in T cell stimulation
• SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation
• Activated B cells express the membrane bound form, the soluble form and the cytoplasmic isoforms of SLAM
• It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells
• Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells
• Moreover, CD150 is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain
• Mutations CD150 gene may be associated with X-linked lympho-proliferative disease (XLP).