IFN gamma Monoclonal Antibody (4S.B3), Biotin, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
IFN gamma
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g
Gene Symbols
IFNG
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
IFNG
Clone
4S.B3
Applications
ELISA
Conjugate
Biotin
Host Species
Mouse
Target Species
Human
Gene Accession No.
P01579
Gene ID (Entrez)
3458
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma)
- Human IFN gamma is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine
- IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells
- Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma
- Glycosylation of IFN gamma does not affect its biological activity
- Applications Reported: The 4S.B3 antibody has been reported for use in ELISPOT, and ELISA
- Applications Tested: The 4S.B3 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human interferon gamma (IFN gamma) in combination with the purified NIB42 (14-7318) or MD-1 (14-7317) antibodies for capture and recombinant human IFN gamma (29-8319) as the standard
- A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 μg/mL
- A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate
- Filtration: 0.2 μm post-manufacturing filtered
- IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines
- IFN gamma is a dimeric protein consisting of two 146 amino acid subunits
- IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa
- On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation
- The biological activity of the IFN gamma homodimer is highly species specific
- Human IFN gamma does not show cross-reactivity with mouse
- IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes
- IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines
- Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway
- IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma
- IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN
- IFN gamma gene mutations are associated with aplastic anemia.