CD119 (IFN gamma Receptor 1) Monoclonal Antibody (2E2), PE, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 2E2 recognizes mouse CD119 also known as IFN gamma receptor1
• CD119 heterodimerizes with IFN gamma receptor 2 (AF-1) forming the Class II cytokine receptor
• The ligand IFN gamma binds IFN gamma receptor 1 subunit but signal transduction transpires via the IFN gamma receptor 2 subunit
• The receptor is involved in host defense and expression is broad including B cells, NK cells, macrophages and dendritic cells as well as other non-hematopoietic cell types
• Applications Reported: This 2E2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 2E2 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• In addition, longer incubations appear to help stain the popμLation; therefore we recommend a 60 minute incubation at 2-8°C
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor
• Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2
• A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection
• In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.