CD265 (RANK) Monoclonal Antibody (R12-31), eBioscience™, Invitrogen™

Description

• Description: The R12-31 monoclonal antibody reacts with mouse RANK (Receptor activator of NF-kappaB), a recently cloned member of the TNFR superfamily with no significant homology to other members of this family
• RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival
• The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6
• Overexpression of RANK activates NF-kappaB and c-Jun-terminal kinase (JNK) pathways
• Recent studies have shown that RANK interaction with TRAF6 activates NF-kappaB, whereas JNK activation is mediated through binding of RANK to TRAF2
• Applications Reported: The R12-31 antibody has been reported for use in flow cytometric analysis
• Applications Tested: The R12-31 antibody has been tested by flow cytometric analysis of mouse RANK transfected cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• RANK is a member of the tumor necrosis factor receptor (TNFR) family
• The full length mouse RANK cDNA encodes a type I transmembrane protein with an extracellular and a cytoplasmic domain
• The extracellular domain contains two potential N-linked glycosylation sites
• RANK shares significant amino acid homology with other members of the TNFR family in its extracellular four cysteine-rich repeats
• Human and murine RANK share 81% amino acid identity in their extracellular domains.