V5 Tag Monoclonal Antibody (TCM5), eBioscience™, Invitrogen™

Description

• Description: This TCM5 monoclonal antibody recognizes recombinant proteins containing a V5 epitope tag
• The V5 epitope is naturally present on the P and V proteins of the paramyxovirus, SV5
• TCM5 specifically recognizes the sequence GKPIPNPLLGLDST
• Applications Reported: This TCM5 antibody has been reported for use in western blotting, and immunocytochemistry
• Applications Tested: This TCM5 antibody has been tested by western blot and immunofluorescent staining of V5-transfected cells and can be used at less than or equal to 1 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available
• The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5)
• The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).