IL-1 beta Monoclonal Antibody (B122), eBioscience™, Invitrogen™

Description

• Description: The B122 antibody reacts with mouse and rat interleukin-1beta (IL-1beta), a 17 kDa factor secreted primarily by monocytes
• IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors
• Applications Reported: The B122 antibody has been reported for use in cytokine neutralization, immunoblotting (WB), and ELISA
• Please refer to Functional Grade purified B122 (cat
• 16-7012) for functional assays
• Applications Tested: The B122 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-1 beta (IL-1 beta) in combination with the biotinylated polyclonal anti-mouse interleukin-1 beta for detection and recombinant mouse IL-1 beta (14-8012) as the standard
• A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL
• A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells
• IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol
• Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals
• Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta
• IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism
• Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions
• IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha
• IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition
• IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury
• Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.