7090447
T-bet Monoclonal Antibody (eBio4B10 (4B10)), PE-Cyanine7, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
T-bet
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
HGNC:11599; LOW QUALITY PROTEIN: T-box transcription factor TBX21; Tbet; T-bet; TBLYM; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor 21; T-box transcription factor TBX21; TBT1; TBX21; T-cell-specific T-box transcription factor T-bet; T-PET; transcription factor TBLYM; transcription factor T-box 21
Gene Symbols
Tbx21
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Tbx21
Clone
eBio4B10 (4B10)
Applications
Flow Cytometry
Conjugate
PE-Cyanine7
Host Species
Mouse
Target Species
Human, Mouse, Rhesus Monkey
Gene Accession No.
Q9JKD8, Q9UL17
Gene ID (Entrez)
30009, 57765, 694044
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet
- T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage
- This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage
- T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling
- In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2
- Moreover, T-Bet plays a role in class-switch recombination in B-cells
- Applications Reported: This eBio4B10 (4B10, 4-B10) antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This eBio4B10 (4B10, 4-B10) antibody has been tested by intracellular stainign and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (cat
- 00-5523) and protocol
- Please see Best Protocol section; (refer to Protocol B: One step protocol for intracellular (nuclear) proteins)
- This can be used at less than or equal to 0.125 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain
- It controls the expression of the TH1 cytokine, interferon-gamma
- Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development
- T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17
- T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression
- Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.