7159122
T-bet Monoclonal Antibody (eBio4B10 (4B10)), eFluor™ 450, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
T-bet
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
HGNC:11599; LOW QUALITY PROTEIN: T-box transcription factor TBX21; Tbet; T-bet; TBLYM; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor 21; T-box transcription factor TBX21; TBT1; TBX21; T-cell-specific T-box transcription factor T-bet; T-PET; transcription factor TBLYM; transcription factor T-box 21
Gene Symbols
Tbx21
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Tbx21
Clone
eBio4B10 (4B10)
Applications
Flow Cytometry
Conjugate
eFluor 450
Host Species
Mouse
Target Species
Human, Mouse, Rhesus Monkey
Gene Accession No.
Q9JKD8, Q9UL17
Gene ID (Entrez)
30009, 57765, 694044
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet
- T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage
- This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage
- T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling
- In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2
- Moreover, T-Bet plays a role in class-switch recombination in B-cells
- Applications Reported: This eBio4B10 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This eBio4B10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product No
- 00-5523-00) and protocol
- Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins located at www.thermofisher.com/flowprotocols
- This may be used at less than or equal to 0.5 μg per test
- T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain
- It controls the expression of the TH1 cytokine, interferon-gamma
- Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development
- T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17
- T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression
- Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.