c-MAF Monoclonal Antibody (sym0F1), eFluor™ 660, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
c-MAF
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
2810401A20Rik; A230108G15Rik; Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; avian musculoaponeurotic fibrosarcoma (v-maf) AS42 oncogene homolog; AW047063; AYGRP; basic domain leucine zipper transcription factor; CCA4; cMaf; C-Maf; c-Maf long form; c-maf proto-oncogene; CTRCT21; fl13d12; hMafA; LOW QUALITY PROTEIN: transcription factor Maf; LOW QUALITY PROTEIN: transcription factor MafA; maf; MAF bZIP transcription factor; MAF bZIP transcription factor A; maf protein; Maf2; MAFA; Mafa homolog; MGC71685; pancreatic beta-cell-specific transcriptional activator; Proto-oncogene c-Maf; RGD1562627; RIPE3b1; RIPE3b1 factor; T lymphocyte c-maf long form; transcription factor Maf; Transcription factor Maf-2; transcription factor MafA; transcription factor RIPE3b1; v-maf avian musculoaponeurotic fibrosarcoma oncogene A; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog a (paralog a); v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A; v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian); v-maf musculoaponeurotic fibrosarcoma oncogene homolog; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; wu:fl13d12; Z-cmaf
Gene Symbols
MAF
Isotype
IgG2b κ
Purification Method
Affinity chromatography
Product Type
Antibody
Clone
sym0F1
Applications
Flow Cytometry
Conjugate
eFluor 660
Host Species
Mouse
Target Species
Human, Mouse
Gene Accession No.
O75444, P54843
Gene ID (Entrez)
17132, 4094
Immunogen
Full-length E. coli-produced c-Maf
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
MAF
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Description
- Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems
- In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific
- More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4
- c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells
- It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well
- Applications Reported: This sym0F1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This sym0F1 antibody has been tested by intracellular staining followed by flow cytometric analysis of TCR-activated mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol
- Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins
- This can be used at less than or equal to 0.125 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- c-maf is the cellular counterpart of oncogenic v-maf that belongs to the family of basic region leucine zipper domain transcription factors
- The leucine-zipper domain is involved in the interaction with LRPICD
- There are two forms of human c-maf mRNA, c-maf-long and c-maf-short
- It is identified in the genome of the acute transforming avian retrovirus AS42
- c-maf targets are IL-4 in Th2 cells, the crystalline genes in lens fiber cells, insulin gene in islet, p53 and L7 where it exerts its transcriptional role through binding to a Maf recognition element (MARE)
- It regulates Th2 differentiation and lineage-specific hematopoiesis
- c-maf is a transcription factor for IL-10 gene expression in LPS-activated macrophages
- Chromosomal aberration involving maf is found in some forms of multiple myeloma
- It is expressed in myeloma cell lines and resting monocytes/macrophages.