Neural/Glial Antigen 2 (NG2) Monoclonal Antibody (9.2.27), Alexa Fluor™ 488, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Neural/Glial Antigen 2 (NG2)
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
4732461B14Rik; AN2; AN2 proteoglycan; AN2/NG2 proteoglycan; chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan NG2; CSPG4; HMW MAA; HMW-MAA; HSN tumor-specific antigen; Kiaa4232; MCSP; MCSPG; MEL CSPG; melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan; MEL-CSPG; membrane-spanning proteoglycan NG2; MSK16; nerve/glial antigen 2; Ng2; Proteoglycan AN2
Gene Symbols
Cspg4
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Cspg4
Clone
9.2.27
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin)
Conjugate
Alexa Fluor 488
Host Species
Mouse
Target Species
Human
Gene Accession No.
Q6UVK1
Gene ID (Entrez)
1464
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: This 9.2.27 monoclonal antibody reacts with human neural/glial antigen 2, which is also known as melanoma chondroitin sulfate proteoglycan (MCSP)
- This antigen is composed of a 250 kDa N-linked glycoprotein and a >450 kDa proteoglycan
- NG2 is present on the surface of >90% of maligt melanomas in addition to some non-melanomic tumors
- Moreover, NG2 is expressed on glioma cells, as well as on developing and adult oligodendrocyte precursor cells
- Studies have demonstrated the involvement of this protein in tumor cell proliferation, adhesion, migration, and invasion
- NG2 also functions as a coreceptor for alpha4beta1 integrin
- Finally, expression of NG2 can be used as a prognostic marker for disorders such as acral letiginous melanoma and infantile acute myeloid leukemia
- The 9.2.27 antibody has been reported to suppress melanoma tumor growth
- Applications Reported: This 9.2.27 has been reported for use in flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded tissue (IHC-P), and immunocytochemical staining of fixed cells (ICC)
- Applications Tested: This 9.2.27 antibody has been tested by immunocytochemistry on methanol-fixed A375 cells at less than or equal to 20 μg/mL and by flow cytometric analysis on A375 cells at less than or equal to 0.125 μg/test
- A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 μL
- A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes
- CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.