Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Alexa Fluor™ 700, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Ly-6A/E (Sca-1)
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
9804; locus A/E; Locus AE; Ly6; Ly67; Ly6a; Ly-6A.2; Ly-6A.2/Ly-6E.1; Ly6A/E; Ly-6A/E; ly6a-e; Ly6e; Ly-6E; Ly-6E.1; lymphocyte antigen 6 complex; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6 complex, locus E; lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; RIG-E; Sca1; Sca-1; Sca-2; stem cell antigen 1; Stem cell antigen 2; TAP; T-cell-activating protein; thymic shared antigen 1; Tsa1; TSA-1
Gene Symbols
Ly6a, Ly6e
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Clone
D7
Applications
Flow Cytometry
Conjugate
Alexa Fluor 700
Host Species
Rat
Target Species
Mouse
Gene Accession No.
P05533, Q64253
Gene ID (Entrez)
110454, 17069
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
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Description
- Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins
- D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells
- Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others
- Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype
- The Ly-6 family is involved in regulation and function of T cell activation
- Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset
- Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Alexa Fluor™ 700 emits at 723 nm and can be excited with the red laser (633 nm)
- Most instruments will require a 685 LP mirror and 710/20 filter
- Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family
- It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice
- Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC
- Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen
- Sca-1 may be involved in the regulation of B and T cell activation.