Ly-6C Monoclonal Antibody (HK1.4), APC-eFluor™ 780, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family
• Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes
• Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes
• These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells
• In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release
• Applications Reported: This HK1.4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HK1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm
• They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments
• In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes
• Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.