7092707
CD8b.2 Rat anti-Mouse, FITC, Clone: 53-5.8, BD
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD8b.2
Concentration
0.5mg/mL
Classification
Monoclonal
Host Species
Rat
Target Species
Mouse
Isotype
IgG1 κ
Purification Method
Affinity Purified
Description
Ly-3.2; Lyt-3.2; CD8b.2
Clone
53-5.8
Applications
Flow Cytometry
Conjugate
FITC
Regulatory Status
RUO
Formulation
Aqueous buffered solution containing ≤0.09% sodium azide.
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Description
- The 53-5.8 monoclonal antibody specifically binds to the β chain of the CD8 differentiation antigen (Ly-3.2 or Lyt-3.2) of most mouse strains, having weak reactivity with Ly-3.1 strains (e.g., AKR, C58, MRL, PL)
- The CD8 α and α chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes
- A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer (the α chain is absent)
- Subsets of γδ TCR-bearing T cells, intestinal intraepithelial lymphocytes, and dendritic cells express CD8a without CD8b
- It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been influenced by thymus-initiated neuroendocrine signals
- CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells
- It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56lck).