7105381

Fibronectin Antibody (SPM539), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

Fibronectin

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human

Gene Accession No.

P02751

Gene ID (Entrez)

2335

Immunogen

Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Clone

SPM539

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Cancer, Cellular Markers, Extracellular Matrix, Mesenchymal Stem Cell Markers, Neuronal Stem Cell Markers, Stem Cell Markers

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

CIGDKFZp686O13149, Cold-insoluble globulin, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, fibronectin, fibronectin 1, FNFINC, FNZ, GFND2ED-B, LETSGFND, migration-stimulating factor, MSF

Gene Symbols

FN1

Isotype

IgG1 κ

Purification Method

Protein A purified

Test Specificity

Fibronectin is a dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This MAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.

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Description

  • Fibronectin Monoclonal specifically detects Fibronectin in Human samples
  • It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.