CD34 Mouse, Clone: QBEnd/10, Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD34
Dilution
Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Primate, Bovine (Negative), Canine (Negative), Rat (Negative), Sheep (Negative)
Gene Accession No.
P28906
Gene ID (Entrez)
947
Immunogen
Detergent solubilized vesicular suspension prepared from human term placenta
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Clone
QBEnd/10
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Adaptive Immunity, Breast Cancer, Cancer, Cell Biology, Cellular Markers, Endothelial Cell Markers, Hematopoietic Stem Cell Markers, Hypoxia, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Myeloid Cell Markers, Neuronal Cell Markers, Neuroscience, Stem Cell Markers, Tumor Suppressors
Formulation
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias
CD34 antigenhematopoietic progenitor cell antigen CD34, CD34 molecule
Gene Symbols
CD34
Isotype
IgG1 κ
Purification Method
Protein A purified
Test Specificity
This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. A
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Description
- CD34 Monoclonal antibody specifically detects Antigen in Bovine (Negative), Canine (Negative), Human, Primate, Rat (Negative), Sheep (Negative) samples
- It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting.