Bcl-2 Mouse, Clone: 124, Novus Biologicals™

Bcl-2

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, SDS-Page

Monoclonal

Purified

RUO

Human, Mouse (Negative), Rat (Negative)

P10415

596

A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

Primary

Store at 4C.

25 kDa

124

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Unconjugated

Mouse

Apoptosis, Autophagy, Cancer, Cellular Markers, Tumor Suppressors

PBS with 0.05% BSA. with 0.05% Sodium Azide

apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2

BCL2

IgG1 κ

Protein A purified

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.