7105678

Cytokeratin, HMW Antibody (KRTH/1076) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

Cytokeratin, HMW

Concentration

1.0 mg/mL

Applications

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Conjugate

Unconjugated

Host Species

Mouse

Target Species

Human, Rat

Gene Alias

CK-2P, cytokeratin 2, Cytokeratin-2P, HUMCYT2A, K2P, K76, keratin 76, keratin, type II cytoskeletal 2 oral, Keratin-76, KRT2Bcytokeratin-2P, KRT2Pkeratin 2p, KRT76, Type-II keratin Kb9

Gene Symbols

KRT76

Isotype

IgG1 κ

Purification Method

Protein A or G purified

Test Specificity

This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Clone

KRTH/1076

Dilution

Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, ELISA, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Formulation

PBS with No Preservative

Gene ID (Entrez)

51350

Immunogen

Recombinant human KRT76 protein

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

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Description

  • Cytokeratin, HMW Monoclonal specifically detects Cytokeratin, HMW in Human, Rat samples
  • It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin, CyTOF-ready.