7105786

Cytokeratin, LMW Antibody (KRTL/1077) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

Cytokeratin, LMW

Concentration

1.0 mg/mL

Applications

Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Conjugate

Unconjugated

Host Species

Mouse

Target Species

Human, Rat

Gene Alias

Cytokeratin LMW, cytokeratin-1B, keratin 77, keratin, type II cytoskeletal 1b, KRT77, type-II keratin Kb39

Gene Symbols

KRT77

Isotype

IgG1 κ

Purification Method

Protein A or G purified

Test Specificity

This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Clone

KRTL/1077

Dilution

Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Formulation

PBS with No Preservative

Gene ID (Entrez)

374454

Immunogen

Recombinant human KRT77 protein

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

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Description

  • Cytokeratin, LMW Monoclonal specifically detects Cytokeratin, LMW in Human, Rat samples
  • It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.