Calponin 1 Antibody (SPM169) - Azide and BSA Free, Novus Biologicals™

Calponin 1

1.0 mg/mL

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Unconjugated

Mouse

Cancer, Cytoskeleton Markers

PBS with No Preservative

1264

Crude human uterus extract

Primary

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

34 kDa

SPM169

Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready

Monoclonal

Purified

RUO

Human, Rat

Basic calponin, calponin 1, basic, smooth muscle, Calponin H1, smooth muscle, calponins, basic, Sm-Calp, SMCCcalponin-1

CNN1

IgG1 κ

Protein A or G purified

Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle