Caldesmon/CALD1 Antibody (CALD1/820) - Azide and BSA Free, Novus Biologicals™

Caldesmon/CALD1

1.0 mg/mL

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Unconjugated

Mouse

Cytoskeleton Markers

PBS with No Preservative

800

Recombinant full-length human CALD1 protein

Primary

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

150 kDa

CALD1/820

Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready

Monoclonal

Purified

RUO

Human, Rat (Negative)

CAD, caldesmon, caldesmon 1, CDMH-CAD, HCAD, LCAD, L-CAD, MGC21352, NAG22

CALD1

IgG1 κ

Protein A or G purified

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.